Knockdown of crbn in zebrafish embryos impaired brain. Sryrelated hmg box sox transcription factors play versatile roles in development and cell fate determination, carrying out as diverse functions as sex determination and craniofacial, neuronal and cardiovascular development. Sox2 regulates muller glia reprogramming and proliferation in the. This antibody recognizes human,mouse,rat, zebrafish antigen. Pdf pleiotropic effects of sox2 during the development of. Confocal immunofluorescent analysis of ntera2 left. Apr 04, 2017 as the vast majority of commercial antibodies are raised against human proteins, we became interested in taking a look at which commercial antibodies are being used by the zebrafish research community. Pdf pleiotropic effects of sox2 during the development.
In the undamaged adult zebrafish retina, sox2 is expressed in muller glia and a subset of. The glofish is a patented and trademarked brand of genetically engineered fluorescent fish. Sox2 antibody detects sox2 protein on zebrafish by whole mount immunohistochemical analysis. It is aimed to help labome visitors find the most suited sox2 antibody. A quantitative rtpcr was performed for mh2a1, mh2a2 and two genes characteristic of undifferentiated stage oct4 and nanog. Due to a lack of a working antibody against zebrafish nestin and to. Validated in wb, ip, ihc, iccif and tested in mouse, rat, horse, chicken, human, pig, xenopus laevis, zebrafish, quail, rainbow trout. The sox2 antibody has been validated for the following applications. Antibodies used in the immunofluorescence assay were polyclonal. The meeting site was beautifully situated in the heart of the minervois wine country, and its remoteness promoted conversations and interaction over the course of the program. In addition the study reports experimental and in silico comparative analysis of jade1 mrna transcripts, jade1 gene structure and analysis of jade1 protein orthologues from mouse human and zebra fish. Significant increases in gene expressions of sox2 up to 2fold, sox3 up to 33fold and sox19a up to 25fold were observed during warming in the embryos that had been chilled for 30. A fresh look at zebrafish from the perspective of cancer research.
Sox2 also participates in growth and renewal on sensory cells in neuromasts of the fish lateral line system, and it is essential for development of taste buds in mammals. Antibody sc 17355 sox17 antibody s 20 by santa cruz biotechnology. Jian xu, in current topics in developmental biology, 2015. Immunoglobulins igs are produced by b lymphocytes and secreted into plasma. The sox2 is a transcription factor involved in adult neurogenesis in different vertebrate species, including fishes. The antibody can be used with either high or low ph antigen retrieval. Monoclonal antibody for studying sox2 in the development research area.
Egfpmi2002, differentiated muller glia express gfp, and in tggfap. Our range of sox2 antibodies can be used for applications including western blotting and elisa please use the filters to sort the attributes in the table below in order to find the antibody that. A cardiomyocyte differentiation in vitro system from zebrafish embryos remains to be established. Sox10 protein staining sox10 antibodies biocare medical. Use the list below to choose the sox2 antibody which is most appropriate for your research. A guide for the laboratory use of zebrafish danio rerio. Making sense of zebrafish neural development in the minervois. Our sox2 antibodies can be used in a variety of model species. Recently, sox2 antibody preparations identified the sox2 protein as a marker for malignant neural gliomas. Retinal regeneration in adult zebrafish requires regulation. Effect of chilling on sox2, sox3 and sox19a gene expression.
Clicking the images or links will redirect you to a website hosted by benchsci that provides thirdparty scientific content. Tissue expression of sox2 summary the human protein atlas. Establishment of novel differentiation models will undoubtedly. Chicken, feline, fish, human, mouse, porcine, primate, rat, sheep.
The zebrafish information network zfin is the database of genetic and genomic data for the zebrafish danio rerio as a model organism. Anti sox2 antibody precisionab polyclonal antibody biorad. To confirm these results, sox2 transcripts were also detected by rtpcr at the oocyte, 2cell, 4cell, 8cell, morula and blastocyst stages figure 1b. Antisox2 antibody clone sox21792 enquire bioreagents.
The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the. Pe antisox2 antibody sox2 is the most studied member of sryrelated box transcription factor family. Sox2 antibody mouse monoclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, enzymelinked immunosorbent assay. Nuclear expression in selected tissues and cell types, including glial cells in brain. Purified antisox2 antibody this rabbit polyclonal antibody recognizes human sox2, also known as sry sex determining region ybox 2 and anop3. Sapphire bioscience genetex gene tex, one of the worlds leading antibody manufacturers, is very proud to provide more than 55,000 quality antibodies including 489 ones validated using zebrafish tissue. Sox10 antibody has been shown to be expressed in 97100% of desmoplastic and spindle cell melanomas and was. The sox2 antibody aids brain cancer research the sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. The zebrafish epithalamus is part of the diencephalon and encompasses three major components. Sox2 is required for maintenance and regeneration, but not. Compare antizebrafish fish antibody products from leading suppliers on biocompare.
Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Validated in wb, ip, ihc, flow cyt, iccif and tested in mouse, human. The purpose of this study was to prepare an antichicken sox2 polyclonal antibody using. Sox2 is essential for formation of trophectoderm in the.
Ihcp image of sox2 ab97959 on zebrafish developing retina sections. These data show that zebrafish sox2 is required for hair cell survival, as well as for. Zebrafish were the first glofish available in pet stores, and are now sold in bright red, green, orangeyellow, blue, pink, and purple fluorescent colors. We use cookies to enhance the usability of our website. However, a complete understanding of the molecules and mechanisms restricting cytosolic bacteria has not been obtained, and the role of bacterial autophagy in vivo remains poorly.
The ig molecule in monomeric form is a glycoprotein with a molecular weight of. Sox2 is an intronless gene encoding a member of the sryrelated hmgbox sox family of transcription factors involved in the regulation of embryonic development and. Sox2 is a widespread marker of pluripotent and many adult stemprogenitor cell types. A variety of different glofish are currently on the market. Sox2 antibody sry sex determining region ybox 2 pser246 biotin details for product antisox2 antibody no. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa.
The sox2 antibody from proteintech is a rabbit polyclonal antibody to a recombinant protein of human sox2. In the developing inner ear, sox2 initially marks all cells in the nascent sensory epithelium and, in mouse, is required for sensory epithelium formation. We maintained fish under standard conditions westerfield, 2000. Please use this form to recommend updates to the information in zfin. Study of pluripotency markers in zebrafish embryos and transient.
Pleiotropic effects of sox2 during the development of the zebrafish epithalamus article pdf available in plos one 91. We showed that sox2 works synergistically with notch. Sox2 is eventually downregulated in hair cells but is maintained in support cells, the. Sox2 was detected in immersion fixed undifferentiated mouse cortical stem cells using goat antihumanmouserat sox2 antigen affinitypurified.
In this study, we used zebrafish as a model to dissect the novel functions of sox2 in the development of the epithalamus. The recovery of injured or lost sensory neurons after trauma, disease or aging is a major scientific challenge. We appreciate as much detail as possible and references as appropriate. Sox2 and sox3 are essential for development and regeneration of. Sox2, a key factor in the regulation of pluripotency and. Pdf developmental changes in the expression of sox2 in. Western blotting with the sox2 antibody on protein extracts from mes cells revealed a single band of 37 kda, the expected size for sox2 protein. Timelapse fret imaging in teen embryos was effective for identifying novel spatiotemporal. Last updated april 5, 2008 zebrafish international resource center zirc 5274 university of oregon eugene, or 974035274, usa phone. The antibody is specific for human, mouse, rat, and chicken oligodendrocyte cell surface marker o4. The image on the left is immunohistochemistry of paraffinembedded human ovarian cancer tissue using csbpa588368sox2 antibody at dilution 115, on the right is treated with fusion. The zebrafish as a new model for the in vivo study of. Online help keyboard shortcuts feed builder whats new. Human orthologs of this gene implicated in breast cancer.
There were significant decreases in sox2 up to 4fold and sox3 up to 3fold expressions following chilling. Zfin provides a wide array of expertly curated, organized and crossreferenced zebrafish research data. Antibodies, primary antibodies, zfish zebrafish, alphabetical listing of zebrafish antibodies. A novel cereblon modulator recruits gspt1 to the crl4crbn. An enhanced green fluorescent protein egfp gene replaces the srybox containing gene 2 sox2 open reading frame in these sox2 gfp knockinknockout mice. Sox2 antibody e4 is a high quality monoclonal sox2 antibody also designated sox2 antibody or sryrelated hmgbox transcription factor 2 antibody suitable for the detection of the sox2. Ntera2 human testicular embryonic carcinoma cell line was stained with mouse antihumanmouse sox2 pe. On the other hand, sox2 plays an important role in neuronal differentiation, but not in pluripotency. Recombinant full length protein corresponding to human sox2 aa 17. Biology and role in development and disease offers a thorough discussion of the important role of sox2 in cellular and developmental processes, aimed at facilitating greater understanding of how sox2 functions across different disciplines.
Antibody staining consistent with rna expression data. The meeting covered neurogenesis and eye development. With the increase in the laboratory use of zebrafish, several advantages, such as a high genetic homology to humans and transparent embryos, which allow clear disease. You may for instance start by entering the antigen name of the target you are interested in. This intronless gene encodes a member of the sryrelated hmgbox sox family of transcription factors involved in the regulation of embryonic development and in the determination of cell. The sections were fixed in formaldehyde and underwent heat mediated. Empire genomics sox2 fish probe is used to detect translocations of the sox2 gene and can be labeled in one of five colors, using standard nick translation protocols.
Everyday low prices and free delivery on eligible orders. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. The epub format uses ebook readers, which have several ease of. Ihcwholemount image of antisox2 antibody ab97959 staining on a mouse embryo. Sox2 antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, flow cytometry fc, enzymelinked. Further analysis of the nestin enhancer revealed an essential sox and. Sox2 is required for maintenance and regeneration, but not initial. Immunohistochemistry with antibody against acetylated. Reliability score i a reliability score is manually set for all genes and indicates the level of reliability of the analyzed protein expression pattern based on available rnaseq data, proteingene characterization data and immunohistochemical data from one or several antibodies with non. The sox10 protein is widely expressed in normal human tissues including melanocytes and breast tissue.
Previous work has shown that sox2 is expressed in the zebrafish. Rabbit anti human sox2 antibody recognizes the transcription factor sox2, also known as sryrelated hmgbox gene 2. This intronless gene encodes a member of the sryrelated hmgbox. Antibody sc 365823 sox2 antibody e 4 by santa cruz biotechnology. We used transgenic fish lines in which muller glia are the only retinal cells that express green fluorescent protein gfp or enhanced green fluorescent protein egfp bernardos et al. Applications key ab array antibody array ae affinity electrophoresis affin chroma. Use the list below to choose the sox2 antibody which is.
Upon hearing loss or balance disorder, regeneration of mechanosensory hair cells has been observed in fish. Is the zebrafish research community using a subset of specialist antibodies raised for zebrafis. Sox2 has been variously implicated in maintenance of pluripotent stem cells or, alternatively, early stages of cell differentiation, depending on context. Previous work has shown that sox2 is expressed in the zebrafish lateral line. Antisox2 antibody products antisox2 antibodies are offered by a number of suppliers. It is also an important marker in malignant tumors such as melanoma, breast carcinoma, gliomas and benign tumors such as schwannomas. Monoclonal antioligodendrocyte marker o4 recognizes oligodendrocyte marker o4 by immunohistochemistry in fixed differentiated rat cortical stem cells, and has also been used in flow cytometry. The book discusses the basic biology of sox2 to help establish the critical foundational knowledge.
Sox2 knockdown in embryonic stem cells results in their differentiation. Sox2 in taste bud and lateral line system of zebrafish. The meeting from sensory perception to motor output. Differential expression of sox17 and sox2 in germ cells and stem. A guide for the laboratory use of zebrafish danio rerio rep spiral by westerfield, monte isbn.
However, despite all the efforts made to establish zebrafish es cell cultures. Dapi was routinely added to the secondary antibody solution at a. Antisox2 antibody staining is expected to be primarily localized to the nucleoplasm. Sox2 is a member of the sox family of transcription factors. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases. Neither the content nor the benchsci technology and processes for selection have been evaluated by us. Developmental changes in the expression of sox2 in the zebrafish brain. This target gene encodes the protein srybox transcription factor 2 in humans and may also be. Transcription factor sox an overview sciencedirect topics. Click on the links to view the chapters or on the attachments below for a downloadable or viewable pdf of the zebra biology book.
Preliminary study on zebra fishs dna exposure to di2ethylhexyl phthalate effects jiatwu lee, hsinghsiao liang department of environmental engineering and science, national pingtung university of science and technology abstract di2ethylhexyl phthalate may interfere with the normal endocrine function of humans and other animals, affecting. Pleiotropic effects of sox2 during the development of the zebrafish. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. A search function with extensive filter options allows you to quickly identify the most suitable product amongst the large product range on. Cereblon control of zebrafish brain size by regulation of neural. Due to their striking genomic similarity to humans, regenerative. Pdf developmental changes in the expression of sox2 in the. Each probe is sold in 20 test kits 20 slides 22x22 mm area and includes hybridization buffer.
Jade1 expression was detected in extraembryonic ectoderm and trophoblast, which are placental components important for vasculogenesis, as well as. Zebrafish antibodies primary antibodies sigmaaldrich. Author summary autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because of its ability to deliver cytosolic bacteria to the lysosome. Pluripotent stem cells, endogenous versus reprogrammed, a.
Send me a copy of this email i agree to the terms and conditions. We at novus biologicals offer a wide variety of highly. Dec 14, 2010 like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and water quality management. Reprogrammed pluripotent stem cells are derived by either somatic cell nuclear transfer scnt whereby there is the transfer of the nucleus from a differentiated cell into the cytoplasm of an enucleated oocyte or by the insertion of specific genes into terminally differentiated cells to artificially induce them to express attributes of more. Detection of sox2 in ntera2 human cell line by flow cytometry. Aug 12, 2015 zebrafish represent a vertebrate model organism that has been widely, and increasingly, employed over the last decade in the study of developmental processes, wound healing, microbehost interactions, and drug screening.
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